Poly(chloromethylstyrene-co-divinylbenzene) (PCD) with 2% cross-linkage is developed as a backbone of immobile artificial enzymes. As the first artificial enzyme built on PCD, an artificial metalloproteinase is prepared by attaching the Cu(II) complex of cyclen as the catalytic center and guanidinium ion as the binding unit. The PCD derivatives prepared were characterized by scanning electron microscopy, IR spectroscopy, elemental analysis, titration of chloromethylphenyl moiety with triethylamine, complexation of p-nitrobenzoate ion to the guanidinium moiety, quantification of the cyclen moieties retaining high affinity for the Cu(II) ion, and determination of log K f for the Cu(II) binding sites. The proteinase activity was measured with γ-globulin (Gbn) by following cleavage of the two chains of Gbn by electrophoresis. The catalytic activity of the Cu(II) complex of cyclen toward Gbn was enhanced by more than 104 times upon attachment to PCD. Gbn complexed to the PCD derivative containing both Cu(II)-cyclen and guanidinium moieties was cleaved by hydrolysis into many pieces with a half-life as short as 10−30 min at pH 4.5−7 and 4 °C. Kinetic data revealed that guanidinium ions attached to PCD acted as effective binding sites for the protein, contributing considerably to the overall catalytic power of the immobile artificial metalloproteinase.

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This paper is to investigate how selection variables and service quality influence on customer satisfaction and loyalty on Korean restaurants. They are chinese living in Korea that compose a little over 50% among foreigners.The findings and marketing guideline are showed up; First, 3 factors among choice attributions had highly significant on customer satisfaction and loyalty. They are 'service', 'food', and 'location' factors. They should reinforce food's taste & quality and also, service staff's elaborate service, and traffic's accessbility. Second, 'sympathy', 'tangibles', and 'response' factors among service quality were strongly influenced on customer satisfaction and loyalty. Therefore, they should provide various menu which is based on seasons & events, food's decoration, and immediate service. Third, customer satisfaction and loyalty are influenced each other. It should strengthen through consistent quality management and service.■ keyword :|Choice Attribution|Service Quality|Customer Satisfaction|Loyalty|

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