The aim of the present work was to extract the seed fat of Rambutan (Nephelium lappaceum L.) for developing nanostructured lipid carriers. Effect of material: solvent ratio and material size time on fat content were investigated. Nanostructured lipid carriers were prepared by melt-emulsification technique. Stearic acid (SA) and the rambutan seed fat (RF) was used as solid and liquid lipid, respectively. Surfactant was Tween 20 as well as Vitamin E was entrapment chemical. Effect of surfactant concentration on formation and stability of solid lipid nanoparticles (SLN) was investigated. At higher surfactant concentrations, NLC had smaller particle size and better stability of nanoparticles. At the optimal concentration of Tween 20 (5% w/w), NLC had a mean particle diameter of 139.43 ± 1.15 nm and a polydispersity index of 0.165 ± 0.017. Zeta potential was above 30 mV.