Purpose: The aim of this study was to investigate the biological and cosmetic properties and the industrial value of the Glycyrrhiza uralensis (G. uralensis) extracts. Methods: The protective effects and anti-inflammatory efficacy of G. uralensis in HaCaT cells and RAW 264.7 cells against anti-oxidation and oxidative stress were analyzed, and the secretion of β-hexosaminidase, which plays a leading role in easing allergic symptoms, was measured. Moreover, anti-bacterial activity on human skin pathogens was measured. Ethanol (70%) and hot water extraction were performed to make the extracts from domestically grown G. uralensis. Results: The comparison revealed that the 70% ethanol extracts were excellent in as antioxidants. Results of the cell experiment using the G. uralensis extracts showed that there was no significant cytotoxicity in HaCaT, RAW 264.7, or RBL-2H3 cells. In HaCaT cells, there was a 28% increase in the protective effects against oxidative stress caused by hydrogen peroxide (H 2 O 2) using a concentration of 100 μg/mL of the 70% ethanol extracts. The anti-inflammatory efficacy of the G. uralensis extracts on RAW 264.7 cells was checked, and it was found that the production of nitric oxide (NO) was inhibited, even low concentrations. The secretion of β-hexosaminidase was also measured, and it was found that G. uralensis and the ethanol and hot water extracts inhibited the degranulation of immune cells, depending on concentrations levels. It was confirmed that G. uralensis 's antibacterial activity was even effective against Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), and Propionibacterium acnes (P. acnes) depending on the concentrations of used. Conclusion: Based on the findings of this study, the G. uralensis extracts are recommended as ingredients for sensitive skin cosmetics. A systematic study on verifying the efficacy of the G. uralensis extracts is further required through tests on humans and animals in order to determine the possibility of its clinical use.