Bacterial control in poultry processing plants is very important, but the swab method for estimating bacterial contamination is somewhat troublesome in routine work. We compared the Desoxycholate Agar Nissui Food Stamp (DA-NFS) based on the agar contact method with the swab method to estimate coliform organisms from various equipments in four poultry processing plants after cleaning. Overall 104 surfaces for coliform organisms were evaluated. The results from 98 (94.2%) surfaces for coliform organisms were equivalent by the DA-NFS and swab methods and there were no significant differences between two methods (P > 0.05). The correlation coefficient between the DA-NFS and swab methods was 0.91. We conclude that the DA-NFS could be useful for routine coliform organisms examination in poultry processing plants after cleaning in Japan.