No abstract

We studied the effects of ethanol on bioamine-containing structures in the jejunum at different stages of alcohol intoxication. The content of catecholamines, serotonin, and histamine in enterocytes of the villus epithelium, submucosa mast cells, crypt enterocytes, and muscular layer was measured by luminescent microscopy and cytospectrofluorometry. Uneven increase of biogenic amine content was found in rats in the initial period of chronic alcohol intoxication (60 days). Further alcohol intake (up to 180 days) impaired the balance of biogenic amines; catecholamines started to prevail.

Study of the effects of 2- and 10-min acupuncture on skin structures in acupuncture points on the upper limb and trunk of outbred albino rats showed that changes in the monoamine status of the skin appeared as early as 15 min after acupuncture. However, the effects in points GV14 and LI11 were different: skin structures in acupuncture point LI11 reacted sooner and more intensively. Acupuncture caused opposite changes in the serotonin index in different structures of the skin in acupuncture points.

The effectiveness of acupuncture contributing to stimulation of morphological organization and altering the dynamics of histamine in the spleen of mice already in the first days after treatment was validated using the methods of luminescent and light microscopy.

The quantitative indicators of neuroamine-producing cells and the content of neuromediators in them were analyzed, the correlation of indicators of their production after bone marrow xenotransplantation was studied. The experiments were conducted on white laboratory mice, which were injected with a cell suspension obtained from a domestic cats into the tail vein. In the early stages of the experiment, the number of neuroamine-producing cells increases, with an increase in the content of neurotransmitters in them. In the subsequent periods of the experiment, their number decreases and the production of all neuromediators (catecholamines, serotonin, histamine) sharply decrease. The formation of lipocytes in the transplanted tissue is showed. Correlations between the levels of neuromediators in neuroamine-producing bone marrow cells also change. Thus, bone marrow xenotransplantation leads to a decrease in the number and production of neuromediators by neuroamine-producing cells within two days.