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The global trend in the production and consumption of food products is the use of food ingredients of natural origin. One of the most valuable sources of biologically active nutrients is pollen of honey bees. Our work is aimed at the development of technology for the production and evaluation of the characteristics of fermented yogurt product with Siberian bee pollen of different terms of collection. To do this, we assessed physico-chemical parameters of bee pollen of the different timing of collection in the apiary s. Kama Kuibyshev district of Novosibirsk region, developed the technology of preparation of dairy products with the addition of bee pollen load; studied the physico-chemical, microbiological and organoleptic characteristics of the obtained dairy products and determined the role of the Siberian bee pollen load different terms of collection in biotechnology yogurt products.

The object of the study was Holstein cows of the second-fourth milk secretion and first-calf cows of different production groups formed depending on the physiological status of the animals. The most pronounced hematological changes were observed in sexually mature cows during the first three months of milk secretion. After fawning, the number of erythrocytes, lymphocytes, and hemoglobin decreased. The concentration of bloodforming elements in the groups with a first-calf cow was significantly higher than in sexually mature cows. The level of erythrocytes and hemoglobin in first-calf cows reaches its maximum value at the beginning of milk secretion. Sexually mature cows after the first month of milk secretion were statistically significantly superior to first-calf cows at the beginning of milk secretion in terms of granulocyte content. Cows, in the first days of milk secretion, had the most fantastic range of variability in the scope of erythrocytes, leukocytes, and hemoglobin, which reflects the individual response of the body to significant changes in physiological status. At the same time, the first-calf cows at the beginning of milk secretion had the most stable level of these hematological indices. Dry cows, as a rule, occupied an intermediate position regarding the variability of the content of blood form elements. Thus, assessing the hematological profile of cows of different production groups helps specialists make targeted adjustments to rations and housing conditions, effectively manage genetic resources and obtain high profits.

In the article, the authors conducted a comprehensive study of the interior, including biochemical, physiological, physicochemical, cytogenetic, and other indicators in sheep of the Romanov breed of Western Siberia. For the study, blood serum samples were taken from 10 rams-producers and 30 lambs at four months. Conventional methods took blood samples. Analyses were performed in the biochemistry laboratory of Novosibirsk State Agrarian University using the technique of competitive enzyme immunoassay with the reagent kit “SteroidIFAtestosterone” for the quantitative determination of testosterone concentration in blood serum. In the sheep breeding area, the authors constantly monitored the content of heavy metals in water, soil, feed, organs, and tissues. The concentration of chemical elements did not exceed the permissible exposure limit. Enzyme-linked immunosorbent assay was performed on a Thermo Scientific Multiskan FC analyzer. The authors performed statistical processing of experimental data using standard methods of descriptive statistics, the statistical programming language, or the RStudio data analysis environment (using the functions DescrStats, Summary, Sd, Read. Table, Write. Table, Aes). The authors established the influence of the age of ontogenesis on the testosterone content in Romanov rams. Presented figure with two variation curves with high transgression. The average testosterone level was 1.8 times higher in lambs (0.774 mmol/l) than in lambs. Testosterone concentration was characterized by high phenotypic variability. The authors have identified reference intervals for testosterone in blood serum depending on environmental conditions in sheep of the Romanov breed of Western Siberia.

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