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We investigate the new simultaneous Absorbance-Transmission and fluorescence Excitation-Emission Matrix method for rapid and effective characterization of the varying components from a mixture. The Absorbance-Transmission and fluorescence Excitation-Emission Matrix method uniquely facilitates correction of fluorescence inner-filter effects to yield quantitative fluorescence spectral information that is largely independent of component concentration. This is significant because it allows one to effectively monitor quantitative component changes using multivariate methods and to generate and evaluate spectral libraries. We present the use of this novel instrument in different fields: i.e. tracking changes in complex mixtures including natural water, wine as well as monitoring stability and aggregation of hormones for biotherapeutics. .