OHdG) levels were estimated at base line (day 0), 1 month, 3 months and 6 months duration. ROS, DFI, 8-OHdG were also assessed in fathers of healthy children. RESULTS: Semen parameters were assessed as per the WHO, 2010 guidelines. The seminal mean ROS levels (p<0.05), sperm DFI (p<0.001), 8-OHdG (p<0.01) levels were significantly higher in fathers of children with non-familial sporadic heritable retinoblastoma as compared to fathers of healthy children. There was a reduction in mean DFI levels post yoga-based lifestyle intervention at 1 month (p¼0.63), 3 months (p¼0.046) and 6 months (p¼0.032). Similarly, there was a reduction in seminal mean ROS levels at 1 month (p¼0.74), 3 months (p¼0.039) and 6 months (p¼0.006). The levels of DNA oxidative base adduct i.e., 8-OHdG were also significantly reduced at 1 month (p¼0.68), 3 months (p¼0.043) and 6 months (p¼0.022) with respect to its base line levels (day 0). CONCLUSIONS: Yoga-based simple lifestyle intervention meditation may significantly lower oxidative stress and oxidative DNA damage, and levels of mutagenic base 8-OHdG in the sperm DNA. Thus, yoga-based intervention is alternative and complementary treatment for maintaining/restoring sperm DNA integrity and hence, can reduce the prevalence of childhood cancers where poor sperm DNA integrity could be one of the reason.