Eleutherine palmifolia (L.) Merr is one of the horticultural that may be utilized as a medicinal plant. It contained flavonoid and anthocyanin. The production of bioactive content on E. palmifolia was affected by light intensity. The objective of this study was to investigate the effect of different shading levels on the physiological response and the yield of Dayak onions. This experiment was conducted at Sukoharjo, District of Ngaglik, Region of Sleman, Yogyakarta, Indonesia, from May to October 2020. This experiment was arranged in a randomized block design non factorial. Three treatments were given, namely no shading, shading 55%, and shading 75%. Each treatment was repeated five times. The result revealed that shade had a varied influence on E. palmifolia’s physiology and yield. Shading 55% and 75% produced the number of tiller and bulb of E. palmifolia lower than no shading. The fresh bulb weight of E. palmifolia in the range between 34.9 – 38.5 g plant-1. The chlorophyll a, chlorophyll b, chlorophyll total, chlorophyll a/b ratio, and chlorophyll b/a ratio content on E. palmifolia’s bulb were not affected by different shading given. Shading 75% gave the highest total flavonoid and anthocyanin content of E. palmifolia’s bulb was 19.77% and 28.10% than no shading, respectively.