Either 50 mg 1(-)tryptophane or 1.0 mg nicotinic acid per 100 gms of ration completely counteracts the growth retardation caused by the inclusion of 40 per cent. corn grits in a low protein ration. A possible explanation of this observation is discussed.

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on liver fat deposition was markedly different-i.e., a marked reduction due to methionine and a small increase due to vitamin B12 (ratios 3 and. 4).Vitamin B12 acted as an efficient supplement to other amino acid combinations, but its physiological mechanism is not evident from this study.The best illustration of the supplementary value of vitamin B12 can be noted in rations 21 and 22. The addition in ration 21 of 0.4% DL-methionine to ration 9 produced a growth of 138.6 grams and a protein efficiency ratio of 2.20, which is less than that secured on ration 9, but the fortification of this ration with vitamin Bi2 in ration 22 not only counteracted the slight amino acid imbalance but was responsible for the production of maximum gain in body weight, 192.8 grams, and an optimum protein efficiency ratio of 2.57. As ration 21 was fortified with methionine, it would appear that vitamin B12, in addition to functioning in synthesis of this sulfur-containing amino acid, increases the utilization of other amino NUTRIENTS IN COFFEE acid combinations as supplements to the proteins in enriched milled wheat flour by a mechanism which will probably be clarified by future research.

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