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A micropropagation procedure was developed to regenerate plants via tissue culture from excised axillary buds of harvested and stored heads of field-grown crisphead (iceberg) lettuce (Lactuca sativa L.). The procedure permits rescue of lettuce germplasm that shows resistance to postharvest physiological disorders and diseases. This procedure was used successfully to regenerate plants of ‘Winterhaven’ lettuce, which showed resistance to russet spot. More than 50% of the excised buds gave rise to viable plants.