StfU, J., Jagos, P., Pokorny, L.: Influence of EDTACAL Spofa Application on Some Biochemical and Hematological Parameters in Sheep Blood. Acta vet. Bmo, 54, 1985: 61-71. In five clinically healthy sheep of the Caucasian merino breed the effect of EDTACAL Spofa application at the dose of 40 mg.kg-1 of the live body mass, applied i.v. once daily in the duration of 5 days, upon the values of 23 biochemical and hematological parameters in blood has been followed. The EDTACAL treatment induced a marked (P < 0.05 -< 0.01) increase of the values of urea, glucose, magnesium (even from the initially hypomagnesiemic levels), blood pH, BE, SB, BB, and on the other hand a marked decrease was seen of the zinc levels (down to the subnormal level), copper, leukocyte count, GMT activity. Moreover, statistically insignificant rise of sodium, potassium, inorganic phosphorus, Hb, % of methemoglobin levels and an insignificant fall of calcium, cholesterol, hematocrit, erythrocyte count, AST activity and later even of sodium level was found. The increase of pC02 values was almost undetectable; insignificant changes were found in the total protein. Following the application of the preparation the values of urea, sodium, magnesium, zinc, copper, pH of blood, GMT activity and of leukocyte count were observed to increase already after 24 hours and of pCOa, BE, SB and BB after two days. The increase in Hb and glucose levels was found after 3 and 4 days, resp. The AST activity decreased only after the cessation of the application. The return to the initial values appeared in most parameters, being established gradually within 2 to 5 days following the cessation of the preparation administration; only the changes in GMT, blood pH and leukocyte count were observed to outlast for further 5 days. The effect of EDTACAL upon the acid-base balance was found to be alkalogenic. EDT ACAL also stabilized some parameters by decreasing the individual daily variations (urea, sodium, calcium, magnesium, zinc, copper, hemoglobin, erythrocytes, leukocytes), and levelled also the differences between individual animals (urea, glucose, sodium, calcium, zinc, erythrocytes and leukocytes). The EDTACAL influence during its application was thus manifested by a broader interference with various metabolic processes in the experimental animal.