New technologies of reintroduction of plant species presuppose implementing both traditional and biotechnological methods for obtaining certain planting materials. However, plants cultivated in vitro exist in specific conditions that lead to changes in their structural and functional state. This explains why it is hard for them to adapt to ex vitro and in situ conditions. Therefore, there is a need for the development of a multistage method of cultivating in vitro plants that would make the influence on their adaptive mechanism in ex vitro and in situ conditions possible. One of its stages is the optimization of the light regime of cultivation which can both initiate the change of the state of the photosynthetic apparatus of plants and increase their bioproductivity stimulating the work of their protective system. This work studies changes in the morphogenesis, growth data and pigment composition of the rare species of Gentiana lutea L. of three populations in the Ukrainian Carpathian (mountains Pozhyzhevska and Sheshul-Pavlyk, plateau Lemska) in vitro focusing particularly on the cultivation light regime. The research has proved the inefficiency of using fluorescent lamps of daylight lamps (LD) type as source of illumination because the low intensity of luminous flux in the area of photosynthetically active radiation (PAR), as well as high proportion of wavelength of blue (400–500 nm) and green (500–600 nm) range in the spectrum cause specific reactions of photomorphogenesis, which, despite the high content of pigments in plastids, lead to poor development of root systems, stretching the stems, formation of small leaves with thin leaflet plate, generally low productivity and low adaptive potential of G. lutea plants to ex vitro and in situ conditions. Complement of cold white light lamps to the fluorescent lamps LD type in the ratio of 1 : 1 enables one to increase the intensity of illumination in the field of PAR and raise the fraction of wavelength of red range (600–700 nm). Such light conditions both improve the bio-productivity of G. lutea plants of all three populations cultured in vitro in comparison to the LD type regimen, reducing the content of chlorophyll b and carotenoids in light-harvesting complexes of photosystems and facilitate an increase in the microclonal multiplication factor without using higher concentrations of exogenous growth regulators,which significantly reduces the cost of the process of obtaining planting materials. It was proved that a combination of LD type lamps, cold white light lamps and phytolamps in the ratio 1 : 1 : 0.6 should be used on the final stages of preparation of the planting material of G. lutea before transferring it to ex vitro and in situ conditions. This relates to the fact that the increase of the wavelength of the red range results in the widening of the active surface of the leaves, rise in the content of photosynthetic pigments, and the noticeable growth of the aboveground and underground parts of the plants. The article assumes that the use of such illumination mode will ensure a faster transition of cultured in vitro G. lutea plants from heterotrophic to autotrophic nutrition, improving their adaptive potential and enabling easier adaptation to non-sterile ex vitro and in situ conditions.

Conditions were developed for microclonal propagation, callus induction and proliferation, plant regeneration, and long-term maintenance of fast-growing normal root cultures of Gentiana species from the Ukrainian flora. The basic growth parameters were evaluated for cultured tissues. Extensive growth and considerable biomass yield was achieved in most cultures. The ability to form tissue and organ cultures depended on the original genotype, type of explant, growth regulator, and mineral composition of the nutrient medium. The efficiency of regeneration from cultured tissue declined with the duration of callus maintenance.

Increasing demand for experts capable of high-quality assessment of the impact of a particular planned activity on the environment involves a more effective use of information and communication technologies (ICT) in the educational process of training ecology students. The authors present a methodology for students to study the educational material of the "Environmental Impact Assessment" course by using ICT. The methodology and algorithm for using the Padlet interactive whiteboard, Mindomo mind maps, Easel infographics, Google Drive and Google Docs in lectures and practical lessons are described. Particular attention is paid to the preparation of a group project using ICT. Tested in the Ternopil Volodymyr Hnatiuk National Pedagogical University, the methodology allows to implement a number of tasks such as: promotion of intensification and effectiveness of teaching; acquisition of practical skills to quickly find the necessary information on various online resources for ecology students; simulation of the real procedure of environmental impact assessment in the classroom; development of the ability to professionally communicate with experts in other fields of knowledge or activity, etc. Examination of the results of using ICT to study the "Environmental Impact Assessment" course has shown a significant increase in informational literacy among ecology students and their individual professional growth.

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The water parameters of the leaves of 9 species of medium-sized trees (Aesculus pavia L., Aralia elata (Miq,) Seem., Broussonetia papyrifera (L.) L’Hér. ex Vent., Cercis canadensis L., Platycarya strobilacea Siebold & Zucc., Prunus dulcis (Mill.) D.A.Webb., Tetradium daniellii (Benn.) T.G. Hartley, Zizyphus jujuba Mill., Robinia viscosa Vent.) and 4 species of bushes (Cotinus coggygrya Scop., Cotinus coggygrya Scop. ‘Royal Purple’, Berberis vulgaris L. ‘Atropurpurea’, Corylus maxima Mill. ‘Atropurpurea’) were investigated in order to identify the most promising drought-tolerant ornamental species of woody plants from the temperate and continental climate zones, which are still not widely cultivated in the Polissia and Forest-Steppe zones of Ukraine. It will be a further recommendation to include them in the ecosystems of the urban environment to overcome the negative impact of the "urban heat island". In order to determine the relative drought resistance of plants, the following parameters of the water regime were measured: tissue hydration and water loss per unit of time. The test for drought resistance was carried out twice, when high temperatures are maintained: in early June (the leaves on trees and bushes are young), and also in early September (the leaves have already acquired all the features characteristic of their species). According to the results of the study, such medium-sized trees as: Aesculus pavia, Aralia elata, Cercis canadensis, Tetradium daniellii and Zizyphus jujube, as well as Cotinus coggygrya, Berberis vulgaris 'Atropurpurea' and Cotinus coggygrya 'Royal Purple' bushes can be recommended for urban landscaping as drought-resistant plants. Whereas, the tree species Broussonetia papurifera, Robinia viscosa, Prunus dulcis and Platycarya strobilacea, as well as the bushes Corylus maxima 'Atropurpurea' showed relatively lower drought resistance, which must be taken into account when landscaping, since, on the one hand, there is a need for additional watering, on the other hand in in one landscape exposure, it is appropriate to plant species with a similar level of drought resistance, which will facilitate further plant care. The slightly lower tolerance to drought of Corylus maxima 'Atropurpurea' may be due to the fact that only this species of the studied shrubs is shade-tolerant, while the other three species are light-loving plants, which must also be taken into account when creating plant compositions.