The objective of this study was to evaluate the efficiency of Bacillus subtilis inoculation in the agronomic performance of the soybean cv. M 9144 RR crop under field conditions. Two field experiments were developed with inoculation by B. subtilis in soybeans in two regions. The experiments were conducted in 2015/2016 harvest from December to April. The experiment was performed in a randomized block design with four replicates with and without inoculation by B. subtilis. Consequently, biomass and productivity were determined. The B. subtilis inoculation increased soybean biomass in both assessment regions. This bacterium showed increased soybean yield in Gurupi and Araguaçu soils (Tocantins, Brazil), with increases above 14.9 and 8.8%, respectively. The inoculation of the strain B. subtilis UFT-Bs10 provided biomass increase, nodulation, stand maintenance and increased yield of soybean cv. M 9144 RR in field conditions.