Lamiaceae members are commonly used in ethno-medicinal practices of our country. The in vitro antibacterial and antifungal activity of various solvents extracts of different aerial parts of Ocimum americanum L., O. basilicum L. and O. sanctum L. wereassessed on standard bacterial and fungal strains using standard laboratory methods. Extracts from O. americanum have inhibitory activity against B. subtilis, B. cereus and S. aureus.Acetone extracts of O. basilicum and O. sanctum were found to be more potent being capable of exerting significant inhibitory activities against majority of the bacteria investigated. Acetone extract of young inflorescence of O. americanum showed highest antibacterial activity against B. cereus (14±1mm) which was also higher (10±1mm) than the inhibition of standard Clotrimazole (10mcg). Hot petroleum ether extract of mature leaves of O. basilicum showed highest activity against E. coli (16±2mm) which was also higher than the inhibition ofAmpicillin, Streptomycitin, Erythromycin.Petroleum ether extract of young leaves of O. sanctum recorded highest inhibition against P. vulgaris (20±2mm). Presence of tannins, flavonoids, saponins, phenols in all the parts of the plants. Use of different parts instead of using whole plant will help in sustainable management of these medicinal plants.
Owing to the ethno-medicinal importance of Mentha arvensis L. the present study proposes in-vitro screening of different aerial parts of the plant. Instead of using whole plant, different parts of a plant can also be used which helps in sustainable management of medicinal plants. Phytochemical, antioxidant and antimicrobial activity study of young and mature leaves, inflorescence and stem of the plant was carried out using standard laboratory methods to determine their efficiency against some bacterial and fungal strains. Inflorescence recorded better extraction of phenol and flavonoid content.
The focus of the present study was to determine the association of the anti-oxidant activity of Costus speciosus (Koen Ex. Retz.) Sm. with the total phe-nolic content and the total flavonoid content. The antioxidant activity of the extracts was quantified by diphenylpicrylhydrazyl (DPPH) assay. The total phenolic content was determined by the Folin-Ciocalteau method and the total flavonoid content was determined by the aluminium chloride method. Among the four extracts of the plant, the ethanol extract exhibited the high-est antioxidant activity and also the highest total phenolic and flavonoid con-tents. A strong correlation was observed between the antioxidant activity and the total phenolic and flavonoid contents. A statistical model was de-rived to explain this dependence and a non-linear association was observed between the antioxidant activity and the total flavonoid content.
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