Transfer ribonucleic acid was hydrolyzed by acid under conditions that released the purine residues. Examination of the hydrolysate by means of ion-exchange chromatography on a sulfonic acid resin revealed the presence of a A number of articles reports the presence of amino acids or small polypeptides, apart from the amino acids attached to the acceptor end of tRNA molecules, bound to nucleic acids. Ingram and Sullivan (1962) and Akashi et al. (1965), for example, have reported the presence of amino acids bound to RNA which cannot be removed through the use of extensive deproteinizing procedures. Balk et al. (1964) and Olenick and Hahn (1964) have reported the presence of amino acids in highly purified preparations of DNA isolated from a variety of sources. The nature of the amino acid-nucleic acid linkage, however, has not been elucidated. Bogdanov et al. (1962) have reported that tRNA contains amino acids attached to the phosphate residues. Harris and Wiseman (1962) have also reported the presence of small polypeptides attached to the

Dame which is operated under contract with the U. S. Atomic Energy Commission. This is AEC Document No. COO-38-476. The author wishes to thank Mr. Robert B. Callen for his aid in the preparation of the diphosphine.(13) The method used is given in S. N. Foner and R. L. Hudson, J.

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