This research studied the use of Artificial Neural Network (ANN) using feed-forward back-propagation model to optimize and predict the performance of a motorcycle fuel injection systems of gasoline. The parameters such as speed, throttle position, ignition timing and injection timing is used as the input parameters. While the parameters of fuel consumption and engine torque is used as the output layer. LavenbergMarquardt model type with train function tanh sigmoid and 25 neurons number is used to generate the target value and the desired output. Variation of ignition timing as optimization variable in a wide range of speed and throttle position is used in experimental tests. ANN is used to investigate the prediction of performance motorcycle engines and compared with the test results. Results showed that the operation of ANN in predicting engine performance is very good. From the test results obtained a smooth contour MAP compared to the initial state. The prediction result and performance test show a good correlation in small error value of training and test that is regression with range 0.98-0.99, mean relative error with range 0.1315-0.4281% and the root mean square error with range 0.2422-0.9754%. This study shows that the feed-forward back propagation on ANN model can be used to predict accurately the performance of a motorcycle engine injection system.