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Liquid chromatography with fluorometric detection has been developed for the determination of the metabolite of coumarin (7-hydroxycoumarin) in human urine. 7-Hydroxycoumarin and 5,7-dimethylcoumarin were separated simultaneously on a 25 cm  4.6 mm i.d. Phenomenex CN 5 mm column with acetonitrile-water (40 : 60, v/v) as mobile phase at 1.0 mL/min, and detected at excitation 325 nm and emission 470 nm. The limit of detection was 0.28 ng/mL for 7-hydroxycoumarin. Analysis of human urine 1-6 days after ingestion of oral Chinese medicines, led to the conclusion that the concentration of 7-hydroxycoumarin was higher than for control urine.