In the past years, various phytosynthetic processes have been utilised of silver nanoparticles for the low cost and environmentally friendly comparing to the chemical and physical methods. These unique metal nanoparticles are used in several applications which are including pharmaceuticals, antimicrobial and biomedical applications. Wherefore, the object of this work is biosynthesizing AgNPs from extracts of pineapple juice as reducing studying their characteristics by Ultraviolet-Visible (UV-vis) Spectrometer, Atomic Force Microscope (AFM), SEM Scanning Electron Microscope and Transmission Electron Microscope (TEM) The UV-Vis spectra results show a strong resonance centered on the surface of silver nanoparticles (AgNPs) at 410 nm. the presenting study is involved the preparation of (AgNPs) from natural source, which confirmed by UV-Visible spectral analysis in 410nm and the synergism effects with some antibiotic to increase the sensitivity to some pathogenic bacterial isolate as E. coli. In addition, our study is presented results of the minimum inhibitory concentration (MIC) that MIC for AgNPs is 25 while other antibiotics are (32,64,128). Finally, the synergism activity shows a significant difference in the sensitivity of some antibiotics used against E. coli bacteria, especially with levofloxacin and increased the inhibition form 419 mm to 1657.2 mm.

The current investigation was intended to assess the viability of sesame oil got from Heet city and Indian premium (saussurea costus) affectability testing against Acinetobacter baumanii isolates detached from disease destination like urine, sputum, C.S.F, wound swab, and burn swab. The results indicated that sesame oil activated the gentamycin antibiotic activity from 3.08 mm to 14.7500 mm and also the Indian premium made the same thing the activation was from 5.4167 mm to 9.3333 mm. while the addition of sesame oil to Imipenem anti biotic there was inhibition in the activity of Imipenem from 21.33 mm to 20.083mm and when we added the Indian premium there was activation to the Imipenem antibiotic was from 22.1667 mm to 23.4167mm. Finally the results of cefotaxime after adding the sesame oil we noticed that there is decrease in the activity of cefotaxime from 3.500 mm to 1.000 mm and we also noticed that the addition of Indian premium to the antibiotic there was decrease in the activity from 3.9167 mm to 3.333 mm. Therefore ower explanation that there is activation to some antibiotics there is also inhibition to the other by adding different plant extract.

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