Chondroitinase ABC (chondroitinase, cABC, ChABC or CHASE) is an enzyme with systematic name chondroitin ABC lyase that specifically acts as chondroitin depolymerizer and also effective on other proteoglycans viz. dermatan sulfate, keratan sulfate and hyaluronate in lowering their levels in irregular extracellular matrix (ECM) formation during cartilage repair process in burns and accident, without disturbing other ECM components. Proteoglycans are the molecules with strong negative charge that attract positive ions ABSTRACTThe rationale behind the microemulsion (ME) formulation was to achieve the efficacy of intralesional delivery and permeability of chondroitinase (CHASE) along with enhanced stability over conventional delivery mode and thus patient compliance. The formulation proceeded with the pseudo-ternary phase diagrams construction to identify microemulsions regions and to establish unique ratio of oil surfactants and aqueous phase. The components used for microemulsion formulation were oleic acid as oil phase, Tween 80 and propylene glycol as surfactantcosurfactant mixture and phosphate buffer pH 7.4 as aqueous phase. The concentration of CHASE in microemulsions was 2.96 U/mL which was stabilised with 1M trehalose solution (w/w). The physicochemical properties of microemulsion were determined. The localization of CHASE within the dermal membrane was determined by in vitro permeability study using excised porcine ear skin. The permeation and the penetration properties of CHASE loaded microemulsion were compared with stabilised aqueous solution of CHASE for varied duration.