Objectives:The objective of this study was to verify the effect of brushing on the mass, color and roughness of the acrylic resin artificial teeth of the RDP, using a dentifrice incorporated with 1% Brazilian Red Propolis (BRP) after 5 years of brushing. Methods: The specimens were distributed in 3 groups / 10 each: Distilled Water (DW), 1% BRP-based dentifrice (PD) and Positive Control dentifrice (CD), analyzed before and after 24, 36 and 60 months of brushing. Data were expressed as mean and standard deviation, submitted to the Kolmogorov-Smirnov normality test and compared by Friedman or Kruskal-Wallis tests followed by the Dunn post test (p <0.05, GraphPad Prism 5.0). Results: It was found that the mass had a statistically significant reduction over time, the color was observed that all experimental groups showed a significant variation (∆E) and roughness did not show significant variations between groups over time. Conclusion: It was concluded that 1% BRP-based dentifrice did not cause deleterious effects on the surface of acrylic resin artificial teeth after 5 years of brushing. However, further in vivo and in vitro studies should be performed to evaluate their long-term use and then confirm their effectiveness in sanitizing RDP.