The intensive development of domestic poultry farming determines the urgent need to achieve the environmental safety of the industry's products, which excludes the introduction of feed antibiotics into the poultry diet. In addition, the existing instability in the market for domestic biological products, whose share is currently no more than 40% and continues to decline steadily, dictates the need to search for approaches to import substitution. An example of a current trend in poultry farming is the use of domestic veterinary drugs of natural origin, which include probiotic feed additives containing cultures of live lactic acid microorganisms, typical representatives of which are enterococci. A wide search for bio-technologically valuable microorganisms among representatives of the genus Enterococcus allowed us to isolate and characterize the Enterococcus faecium ICIS 96 strain, which is promising for creating a probiotic feed additive. It is known from the literature that lactic acid microorganisms are able to regulate the metabolism of poultry. It is appropriate to assume that the culture of E. faecium ICIS 96, when introduced into the diet of poultry, can have a beneficial effect on energy metabolism. Clarification of this assumption determined the purpose of our work.