The association between advanced paternal age and impaired reproductive outcomes is still controversial. Several studies relate decrease in semen quality, impaired embryo/fetal development and offspring health to increased paternal age. However, some retrospective studies observed no alterations on both seminal status and reproductive outcomes in older men. Such inconsistency may be due to the influence of intrinsic and external factors, such as genetics, race, diet, social class, lifestyle and obvious ethical issues that may bias the assessment of reproductive status in humans. The use of the murine model enables prospective study and owes the establishment of homogeneous and controlled groups. This study aimed to evaluate the effect of paternal age on in vitro embryo development at 4.5 day post conception and on in vivo fetal development at 16 days of gestation. Murine females (2–4 months of age) were mated with young (4–6 months of age) or senile (18–24 months of age) males. We observed decreased in vitro cleavage, blastocyst, and embryo development rates; lighter and shorter fetuses in the senile compared to the young group. This study indicated that advanced paternal age negatively impacts subsequent embryo and fetal development.
Trachemys scripta elegans é uma espécie de tartaruga semiaquática, de água doce, nativa da América do Norte. Ela é conhecida popularmente como “tartaruga de orelhas vermelhas”, por possuir duas manchas vermelhas, uma em cada lado da cabeça. No cativeiro espécies aquáticas são mais propensas ao aparecimento de doenças fúngicas e bacterianas, especialmente se o manejo alimentar e sanitário não for adequado. Umidade elevada no ambiente pode causar amolecimento da queratina e das placas córneas, facilitando o processo de invasão por fungos, em casos extremos, pode levar ao óbito do animal. As infecções fúngicas podem acometer qualquer parte do corpo, incluindo a pele, trato respiratório, estômago, intestinos, fígado, rins e baço. Diante disso o objetivo determinado desse artigo é de realizar a microcultura da carapaça dessa espécie e constatar os achados fúngicos, onde foi possível demonstrar a importância de um manejo adequado para assim ser evitado novas infecções e a resistência fúngica.
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