No abstract

Materials and Methods Reagents, solvents and materials Thymol (99.5 % purity), PLGA (Poly D, L-lactide-co-Glycolyde 50:50) and poloxamer 188 (Poly(ethylene glycol)block-poly(propylene glycol)-block-poly(ethylene glycol) were purchased from Sigma Aldrich (São Paulo, USA), acetonitrile grade HPLC and acetone grade P.A. were purchased from Tedia (New Janeiro BR), water purified through milli-q filter system (Milipore, Brazil), phosphatidylcholine (Lipoid S-100) were purchased from Lipoid (Newark, USA), essential oil of Lippia sidoides (OELS) was purchased from Padetec (Fortaleza, BR), Millex-LCR filter with PTFE membrane modif. 0,45μm was purchased from Milipore (São Paulo, BR). Instrumentation The chromatographic analyzes were performed on a liquid chromatography (Waters Alliance 2695, USA) with diode array Volume 10 Issue 2-2017