No abstract

PLA2 dependence for ERK 1/2 activation in response to cyclic stretch in proximal tubular epithelial cells was established in this report. In addition, these findings indicate cyclic stretch increased the tyrosine phosphorylation of the EGF receptor and c-Src and that c-Src acts upstream of the EGF receptor to mediate its phosphorylation, whereby both are critical for stretch- induced ERK 1/2 activation in rabbit proximal tubular cells. These observations documents for the first time a mechanism of mechanical stretch-induced kinase activation mediated by stretch activated Ca2+ channels and PLA2-dependent release of arachidonic acid.

In renal proximal tubule epithelial cells, a membrane-associated phospholipase A 2 (PLA 2 ) is a major signaling pathway linked to angiotensin II (Ang II) type 2 receptor (AT 2 ). The current studies were designed to test the hypothesis that membrane-associated PLA 2 -induced release of arachidonic acid (AA) and͞or its metabolites may serve as an upstream