A 5-year-old girl had a "flu-like" syndrome followed five days later by clinical and pathologic evidence of myositis localized to both calves. Serum complement fixation titers were compatible with influenza A viral infection. The illness improved spontaneously within a week. Possible pathogenic mechanisms of postinfluenzal myositis and its clinical differences from idiopathic polymyositis are discussed. (29:441-443, 1973)
July 25, 1989; accepted February 26, 1990. demonstrated increased deposition of [3H]fluoromisonidazole in experimental myocardial ischemia.7[18F]Fluoromisonidazole has been successfully synthesized, and preliminary studies using positron emission tomography (PET) in dogs have shown enhanced regional deposition of this tracer in ischemic myocardium.8,9To study the relation between myocyte Po2 and fluoromisonidazole uptake, we measured the uptake and retention of [3H]fluoromisonidazole in isolated adult rat myocytes, because this model allows more precise control of cellular Po2 than can realistically be achieved in vivo. The results indicate a direct relation between reduced cellular P02 and enhanced fluoromisonidazole uptake in cardiac myocytes.
Materials and MethodsIsolated, calcium-tolerant adult rat myocytes were obtained by collagenase dissociation.10 Hearts were rapidly excised, mounted on a Langendorff apparatus, and perfused for 1 minute at constant pressure (85 mm Hg) with calcium-free modified minimum essential medium (GIBCO Laboratories, Grand Island, N.Y.) supplemented with insulin, taurine, and creatine. All buffers were prewarmed to 370 C, equilibrated with 95% 02-5% CO2 at pH 7.4, and adjusted for osmolarity. After the initial perfusion cleared blood from the heart, perfusion continued for another 15 minutes with medium containing 0.06% by guest on
The effect of aging on the levels of dopamine (DA) in striatum and noradrenaline (NA) in cerebral cortex, cerebellum, hypothalamus, midbrain and pons-medulla from young adult and five groups of aging rats was evaluated. Determinations of the levels of these amines were conducted. Age-related changes which commenced at 12 months were observed in specific brain regions. The endogenous content of NA decreased significantly in hypothalamus, midbrain, and pons-medulla when compared with the same regions from young adult animals. These results show that central noradrenergic system is markedly vulnerable to the aging process in rats.
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