The diabetic mellitus (DM) disease is a danger risk and a cause for rising public health anxietyat both develop and non-developing countries. T2DM is described by insulin resistances and/or abnormal insulin secretions from pancreas. Dehydroepiandrosterone sulfate: is a significant mammalian steroid hormone, the synthesis of this hormone from pregnenolone in the body. DHEA is a source of production of other hormones that the body need like estrogens and testosterone. Objectives: To study Serum DHEA-S hormone estimationas predictor of T2DM in relative with other limitations in diabetic patient. Material and Method: the study designed as case control experimental study was conducted on diabeticpatient at Al-Seder Hospital at AL-Najaf City-Iraq from February to April 2020. Results: the results of this study show there is significantly differences in (p< 0.05) between T2DM patients and control group, there is significant decrease in DHEA-S hormone levels at patient, while in regarding to thyroid hormones the T3 and T4 were significantly decrease on other hand TSH was increased. The lipid and sugar profiles were significantly increased at (P< 0.05). Conclusion: our study conducted There is a main relationship between DHEA-S hormone level and T2DM. This supports that DHEA-S level is reversely related with the diabetes progression.