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In enzymatic lysis of yeast for the recovery of intracellular proteins, the rupture of whole cells is caused by the action of a lytic system consisting primarily of protease and glucanase. A first-principles mechanism for the lytic reaction based on a two-layer model of the wall structure and a burst model for the disruption of cells is pre sented. The fed-batch model results in a dynamic optimization problem, with the enzymes, activities being the control variables. Orthogonal collocation is applied to discretize the state equations, and the resulting non linear program is solved using successive quadratic pro gramming to determine the enzyme and protease inhibitor add-in rates and pH control profiles that maximize the recovery of intracellular protein. Applying the proposed approach, optimal profiles were determined such that a significant increase of the production of proteins in a fed-batch reactor is realized. Also, the optimal control policies in a series of continuous-flow stirred tank reactors (CFSTRs) are determined.

The work is aimed to investigate the mechanical responses of bare dies of the combination of pure tin∕Al–NiV–Cu Under bump metallization (UBM) and packages of pure tin∕Al–NiV–Cu UBM/substrate of standard thickness of aurum. The mechanical properties under multiple reflow and long term high temperature storage test (HTST) tests at different temperatures and the operational life were obtained. A scanning electron microscope was used to observe the growth of IMC and the failure modes in order to realize their reaction and connection. From the empirical results of bare dies, the delamination between IMC and die was observed due to the tests at 260 °C multiple reflow. However, their mechanical properties were not affected. Nevertheless, the bump shear strength of bare dies were decreased by HTST tests. In package, all the results of mechanical properties by multiple reflow test and HTST test were significantly lowered. It was shown that the adhesion between bump and die reduced obviously as tests going on. As for high temperature operational life test in the conditions of 150 °C and 320 mA (5040A∕cm2), the average stable service time of the package was 892 h, and the average ultimate service time of the package was 1053 h.

In order to reduce energy loss in motor, applications of high-efficiency non-oriented electrical steel sheets and optimal laminating process are both important elements. The motor core loss deterioration is influenced by a number of factors, such as flux distribution, stress and strain, space harmonics, temperature, and short circuits between lamination. In conventional clamping method, steel sheets are laminated via interlocking or welding in general manner. The measured energy loss by welding was much larger than that by interlocking. Therefore, interlocking is well known and usually employed with benefit of easy conducting. The protuberance shapes affected the fastening strength. Generally, the intensity of rectangular type is stronger than the circular counterparts. However, the circular interlocking has better magnetic characteristics. To clarify the method effectiveness, interlocking performances regarding fastened strength and magnetic deterioration by lamination were investigated. The key parameters of protuberance shape and forming depth were designed. Precisely manufacturing operation was applied to avoid interlocking failure. Magnetic properties largely influenced by clamping method are crucial to minimizing the magnetic deterioration during laminating procedure. Several experiments for various processing conditions were undertaken, and the quantification results showed the rectangular interlocking had better fastened strength but worsened iron loss comparing with the circular arrangement. To acquire the comprehensive mechanical and electrical identities for electrical steel lamination, deliberate producing conditions regarding minimizing the magnetic deterioration should be adopted prudently.

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