Gynura cusimbua (D. Don) S. Moore is a favorite food vegetable and traditional folk medicine. The chloroplast genome information, of G. cusimbua , was introduced and released in this study. The complete chloroplast genome was characterized as 156, 684 base pairs (bp) in length. The circle gDNA contained four segments, namely LSC (large single copy), SSC (small single copy) and two IRs (inverted repeats), which was 86, 834 bp and 18, 414 bp and 25, 718 bp in length separately. The total GC content was 36.88%. A total of 125 genes were characterized in the chloroplast genome, where 84, 33 and 8 genes were for coding-proteins, tRNA and rRNA respectively. The phylogeny tree demonstrated that G. cusimbua was clustered with Jacobaea valgaris and Senecio valgaris . This study would fill a vacancy of chloroplast genome information involving G. cusimbua , and provide new genetic resources for the study on Senecioninae .

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