Increased public awareness for a healthy diet must be balanced with successful cultivation. Organic farming system is the right choice since it leaves all non-organic components. This study aims to identify the chemical and microbiological properties of agricultural soils that apply organic systems located in Batang Buluh Village, Pematang Johar, Deli Serdang Regency, North Sumatra. Chemical analysis was carried out at Socfindo Laboratory in Medan, including testing the pH of H2O, total P and K, C Organic, N Kjehldahl, and CEC (Cation Exchange Capacity). Soil microbiological tests were carried out at Medan Regional Health Lab including gram staining tests and biochemical tests. The results of chemical analysis from the analysis of pH H2O, P and total K, Organic C, N Kjehldahl, and CEC (Cation Exchange Capacity) at the edges and middle each obtained that was pH 5 -6, soil total P content was 0.0260% up to 0.450%, available K analysis, namely 0.200% and 0.210%, organic C content obtained results of 0.970% and 0.630%, N content using the KJehldahl method was 0.150% and 0.090%, and CEC obtained results of 14.330 me/100 and 10.090 me/100 g. Related species of Bacillus contained in the soil were Gram-positive, rod-shaped, spore-forming, aerobic or facultative anaerobic, motile bacteria with peritrichous flagella.

Research has been carried out to analyze the content of minerals, fat and protein in salted boiled pufferfish samples. Samples were purchased from a traditional market in Medan City, Indonesia. The method used is the 2005 AOAC method. Crude protein analysis was carried out by the Kjeldhal method, crude fat analysis was carried out by the Soxhlet extraction method, water content analysis was carried out by the drying method, ash content analysis was carried out by the combustion method at high temperature, and mineral analysis was carried out by using an Atomic Absorption Spectrophotometer (AAS). The results of the analysis of the salted mackerel sample obtained crude protein content of 65.9770 %, this indicates that the salted boiled mackerel sample has a high protein content. Crude fat content from the analysis is 16.7600 %. The water content and ash content of the boiled mackerel samples were 9.5077% and 6.2700%, respectively. Mineral analysis, namely the levels of calcium and sodium obtained respectively 0.7635 ppm and 0.7571 ppm.