BALOUNOVÁ MARTA, VACULOVÁ KATEŘINA, HLOŽKOVÁ LENKA, MIKULÍKOVÁ RENATA, EHRENBERGEROVÁ JAROSLAVA: The eff ect of the changed amylose and amylopectin ratio on the selected qualitative parameters in spring barley (Hordeum vulgare L.) grain. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 3, pp. 577-585 In 2008-2011, grain yield and chemical composition were assessed in a set of spring barley genotypes with standard and changed amylose/amylopectin ratio (non-waxy and waxy, respectively) at two locations (Kroměříž, Žabčice). Genotypic as well as environmental eff ects were important for the variations in contents of chemical compounds. The waxy genotypes had statistically signifi cantly higher levels of -glucans and protein (6.9% d.m., 12.1% d.m., respectively) than genotypes with the standard starch character (5.4% d.m., 11.5% d.m., respectively). The hull-less waxy genotype KM2619.413.4 was statistically signifi cantly diff erent from all other genotypes in the set due to its highest content of -glucans (10.80% d.m.). We found statistically signifi cant diff erences between genotypes divided by the type of grain (hulled vs. hull-less) and also by the starch character (standard vs. waxy) in average starch contents as follows: hull-less genotypes with the standard starch composition (64.8% d.m.) > hull-less waxy genotypes (63.7% d.m.) > hulled genotypes with standard starch composition (61.4% d.m.) > hulled waxy genotypes (60.0% d.m.).