Studies were conducted to determine the effects of the whey protein ␤-lactoglobulin (blg) on the intestinal development and intestinal morphology in neonatal piglets. Two trials (5 and 28 days) were conducted on piglets in three separate groups. One group served as a control group while the remaining two were experimental groups based on diets. The two experimental diets were a bovine colostrum powder, one with supplementation with blg and the other without. The control group remained suckling on a sow. All groups were weaned on day 20 and placed onto a solid commercial piglet diet. Enzymatic activity, total DNA synthesis, crypt depth, and villus height were all parameters used to evaluate the effect of blg. Piglets fed a diet supplement with blg had the greatest total DNA after 5 days. Sowreared piglets had the greatest intestinal morphology development with regards to villus height. No significant differences were found in enzymatic activity between treatments. Anat Rec Part A, 288A:601-608, 2006.