Resumo:A piometra é um processo inflamatório do útero, caracterizado pelo acúmulo de secreção purulenta na luz uterina resultante do acumulo de liquido devido a hiperplasia endometrial cística (HEC) somada a uma infecção bacteriana. Acomete várias especies, sendo mais comum em cadelas adultas de meia idade, o quadro do animal pode se agravar rapidamente, sendo uma das afecções que mais causam morte em cadelas, principalmente em decorrencia de endotoxemia e peritonite. O diagnóstico consiste em exame clinico, laboratorial e de imagem. O ultrassom é uma ótima ferramenta diagnóstica para se fechar diagnóstico, principalmente nos casos de piometra fechada. O objetivo deste trabalho é relatar o caso de uma cadela com piometra fechada. O animal apresentou piora no quadro em poucas horas, sendo submetido a cirurgia para tratar essa afecção, durante cirurgia foi constatado peritonite. O tratamento cirurgico se mostrou o mais indicado, apresentando uma boa recuperação pós cirúrgica. O animal voltou a se alimentar e já não se mostrava apático nos dias posteriores a cirurgia. Palavras-chaves: piometra, hemometra, ovariosalpingohisterectomiaAbstract: The pyometra and an inflammatory process make uterus, characterized by accumulation of pus in the resulting uterine fluid accumulation of light due to cystic endometrial hyperplasia (HEC) added to bacterial infection. It affects several species, being more common in female dogs adult Middle-aged, the animals of table may worsen quickly, being one of the conditions que more in bitches cause death, mainly due to endotoxemia and peritonitis. The diagnosis consists of clinical examination, laboratory and image. Ultrasound is a great diagnostic to close close diagnosis, especially in the case closed pyometra. The objective this work and report the case of a bitch with a closed pyometra. The animal worsened in the table in a few hours and underwent surgery to treat this condition during surgery was found peritonitis. The surgical treatment proved the most suitable , with a good post-surgical recovery. The animal returned to feed and no longer showed lethargic in the days after surgery.

Background: Hermaphroditism or intersex is a general term that includes various congenital anomalies of the genital system which is used to define animals with ambiguous sexual characteristics. It occurs in domestic animals, more commonly in pigs and goats, and rarely in horses, dogs, sheep, and cattle. The prevalence of hermaphroditism varies a lot among breeds and species and is higher in groups with a high degree of consanguinity. Therefore, the objective of this report is to describe a case of canine hermaphroditism in a dog with male phenotype, as well as the anatomical and hormonal findings, and classification of the hermaphroditism exhibited by the animal studied.Case: A 1-year-old, mongrel, 5 kg dog was referred to the UHV-UECE due to the presence of a slit on the lower quadrant of the abdomen, caudal to the umbilical scar. At examination, the animal exhibited normal rectal temperature, no alterations of palpable lymph nodes, and a satisfactory body condition score. The pubic area had 2 testicles, each one in a different scrotum, 1 to the right and 1 to the left of the slit. A prepuce with no apparent function was present cranially to the slit, closer to the umbilical scar. At the other extremity of the slit, on the pubic region, there was a flaccid structure similar to a penis (micropenis) with no penile bone and no function. The slit was open until the area ventral to the anus, where the urethra was detected. The animal exhibited a behavior of territory demarcation with urine typically seen in male dogs. Orchiectomy and slit correction surgery were performed. Pre-surgical exams included: complete blood count and hormonal doses of estradiol, testosterone, and progesterone. Abdominal ultrasound was performed to assess presence of sexual glands and gonads. The hormonal exam revealed: estradiol 56.39 pg/mL; testosterone 127.9 ng/mL; progesterone 0.892 ng/mL. A uterus was not detected on ultrasound examination. Ovaries were seen on their typical anatomical position; they were symmetrical and had normal sizes. There was a normal size prostate in the pelvic area, exhibiting normal texture and echogenicity. No other abnormalities were seen and the owner opted for no further surgical intervention.Discussion: Testosterone predominance explains the male behavior and appearance, demonstrating that the testis were prevalent over the ovaries. The occurrence of XX males has been reported. The genetic cause is the absence of the SRY chromosome, which has a fundamental role on activation of the SOX gene, which is responsible for sex determination. Clinically, a true hermaphrodite can exhibit different degrees of genital ambiguity; they can be diagnosed during puberty with the emergence of heterosexual characteristics, or as an adult, with infertility or gonadal neoplasia. True hermaphrodites are individuals with testicular and ovarian tissues, either combined in one gonad (ovotestis) or present as two separate gonads. The presence of ovaries and testicles can be confirmed by histology, which was not performed in this study. However, ultrasound findings (prostate and ovaries), and the presence of normal testis and external genitalia without a defined penis or vulva are in accordance with the description of a true hermaphrodite.

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Background: Sperm sexing is increasing in use because pre-determining the sex of the calf allows greater profitability and promotes significant gains in the productive systems that utilize the technique. Deployment of a low-cost and practical preservation methodology may further favor the cost-benefit ratio. Flow cytometry, the most commonly used sexing technique, has high costs and is very restricted. As an alternative, immunosexing has been studied, which uses sex-specific monoclonal antibodies. Thus, the objective of this study was to evaluate the immunosexing technique in conjunction with cryopreservation in ACP-102c and examine its economic aspects with regard to ram semen.Materials, Methods & Results: Ejaculates from two ram individuals were collected with the aid of an artificial vagina, evaluated, and submitted to the immunosexing protocol, according to the manufacturer's recommendations, using the Monoclonal Antibody Kit specific for mammalian sperm with “Y” chromosomes (HY; HY Biotechnology, Rio de Janeiro-RJ, Brazil). After sexing, the supernatant was resuspended in the cryopreservation diluent: ACP (ACP-102c + 20% egg yolk + 7% glycerol), packaged in 0.25 mL straws, refrigerated to 4°C, stabilized for 30 min, frozen in liquid nitrogen vapors (-60°C) for 15 min, immersed in liquid nitrogen, and stored in cryogenic cylinders. The samples were thawed and evaluated for sperm kinetics both by using computerized semen analysis with SCA® software (Sperm Class Analyzer version 5.0) and subjectively comparing specimens from the two animals using conventional microscopy (40x). Plasma membrane integrity (IMP) and sperm cell morphology were evaluated by the smear staining technique using eosin-nigrosine dye, and the percentages of healthy and normal spermatozoa were determined. A bibliographic survey and a market study of similar products and technologies were carried out to provide an economic viability metanalysis of the bioproduct (ACP-102c) and bioprocess (immunosex). The data were analyzed using R-project©, and comparisons made between animals and between thawing periods using T test. There were no statistically significant differences between animals and between periods (P > 0.05), except for the normal sperm parameter, in which animal A1 had the lowest percentage (P < 0.05). As for the cost-benefit ratio, flow cytometry as a technique is more laborious and expensive, while immunosexing associated with cryopreservation in ACP-102c diluent has proven more practical, with regards to both sperm sexing techniques and diluents for sperm conservation.Discussion: In general, the quality of cryopreserved sexed semen was lower than that of non-sexed semen; however, in this study, both in the comparison between animals and between evaluation periods, similar values of motility, viability, and sperm morphology were obtained for sexed and several non-sexed cryopreserved semen samples, demonstrating that immunosexing did not severely affect the sperm structure, and that the ACP-102c conservation medium was efficient at maintaining the plasma membrane of these sperm. In the evaluation of economic aspects, it was observed that immunosexing, associated with cryopreservation in ACP-102c diluent, proved to be the most practical technique, requiring only conventional equipment, and allowing a greater field of application, since the immunosexing semen can be used for primiparous and multiparous females. Thus, it was concluded that immunosexing associated with cryopreservation in an ACP-102c diluent was more cost-effective, more practical, and had significantly improved sperm quality results after sexing.