Heat shock protein 60 (Hsp60), a typical mitochondrial chaperone, is associated with progression of various cancers. However, its expression and significance in hepatocellular carcinoma (HCC) remain largely unclear. In the present study, the mRNA and protein expression of Hsp60 in HCC tissues were detected by quantitative RT-PCR (n=24), western blot (n=7), and immunohistochemical staining (n=295), respectively. The correlation between Hsp60 expression and clinicopathological characteristics of HCC patient was also analyzed. Meanwhile, the influence of Hsp60 on malignant phenotype of HCC cells was further investigated. We found that expression of Hsp60 was significantly downregulated in HCC tissues compared to peritumor tissues. Hsp60 expression was significantly correlated with serum alpha -foetoprotein (AFP) level and tumor differentiation grade. Moreover, high Hsp60 expression cancer/ pericancer (C/P) ratio was associated with a better overall survival rate (P=0.035, n=295). The prognostic implication of Hsp60 in HCC was further confirmed in another cohort of 107 HCC patients (P=0.027). Up-regulation of Hsp60 remarkably induced the cell differentiation and inhibited the invasive potential of HCC in vitro and in vivo. Intriguingly, the down-regulation of Hsp60 significantly impaired mitochondrial biogenesis. Although more data are required to clarify the underling mechanism responsible for function of Hsp60, our results suggested that the effect of Hsp60 on differentiation and invasion of HCC cells might be associated with mitochondrial biogenesis. Collectively, our findings indicated that Hsp60 exerted a tumor suppressor function, and might serve as a potential therapeutic target in the treatment of HCC.

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For the most advanced storage system which is still in study, the high-speed network bandwidth is a key target of the system. And the measurement on the super-high network bandwidth is one of the most important works in the process of evaluating the completely new developed massive storage system. However, sometimes we can't find a single node available to produce sufficient load to test such a high-speed bandwidth, with this situation of single node's limitation, based on TCP protocol, we propose a new and simple strategy, which uses multiple nodes to produce high bandwidth load by constructing a cluster, to test the high-speed bandwidth parallelly. This paper demonstrates the feasibility of this strategy and analyzes main parameters of TCP protocol which affect the link bandwidth significantly by a series of experiments; at last we also put researches on the performance boost of bonding NICs with multiple NICs in single node. Finally, we give a solution to implement the high-speed bandwidth measurement.

Researches on technologies about testing aggregate bandwidth of file systems in cloud storage systems. Through the memory file system, network file system, parallel file system theory analysis, according to the cloud storage system polymerization bandwidth and concept, developed to cloud storage environment file system polymerization bandwidth test software called FSPoly. In this paper, use FSpoly to luster file system testing, find reasonable test methods, and then evaluations latest development in cloud storage system file system performance by using FSPoly.