In this paper, the antibacterial property of hemp fiber was studied before and after extracted with ethanol. Simultaneously, the chemical composition of ethanol extract from hemp fiber was analyzed and characterized by UR and FT-IR. Experiment results show that, the antibacterial property of hemp fiber decreased after extracted with ethanol. The Inhibitory rate to Candida albicans decreased from85.7% to 64.4%. The Inhibitory rate to Staphylococcus aureus, Escherichia coli and Candida albicans deceased as extraction time prolonging, and attained equilibrium after about 4 hours. Through experiments and date analysis by using FT-IR and UV, hemp fiber contains chalcone. It is initially argued that the active anti-bacterial constituents of hemp fiber may be alkaloids, flavones and saponins.

The hemp-stalk powders exhibit a certain degree of antibacterial properties, the antibacterial rate is about 41% against Staphylococcus aureus, about 74% against E. coli, about 50% on against Candida albicans respectively. Extract the hemp-stalk powders with petroleum ether, ethyl ether, ethanol, 75% ethanol, water, the order of solvents were ranked by the polarity. The effective antibacterial ingredients in hemp could be extracted with petroleum ether, ethanol, 75% ethanol by the horizontal comparison of antibacterial rates and antibacterial changing rates.