Cisplatin-treated rats experienced a significant elevation of serum activities of lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT) and creatine kinase (CK). These effects were accompanied by significant increases in the levels of malondialdehyde (MDA) and nitric oxide (NO), and decreases in the glutathione (GSH) content, and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in cardiac tissues. Pathological examination revealed that cisplatin caused significant cardiac damage in rats.Grape seed proanthocyanidins extract (GSPE) administration produced amelioration in biochemical indices of cardiotoxicity, lipid peroxidation, oxidative stress, as well as histological change when compared to group cisplatin alone. GSPE were shown to be potential candidates to ameliorate cisplatin-induced cardiotoxicity.