Objectives: Assess the diagnostic value of Bone Metabolism Biomarker Osteocalcin for Monitoring Treatment Chronic moderate Periodontitis. Materials and Methods: 84 patients with chronic moderate periodontitis were participated in this study who received periodontal therapy. All patients underwent a thorough clinical and laborator examination including a determination of the serum osteocalcin content, at the beginning of the study and 1, 3 months after therapy. Results: Healing period occurred without complications, and with minimal postoperative discomfort. The mean BOP before treatment was 2.5 ± 0.31, after 1 month treatment the mean BOP 1.7 ± 0.1, after 3 months treatment the mean BOP 1.3 ± 0.12(p>0.05). The probing depth according to the mean value (PD) was 5.27 ± 0.77 mm at the beginning of the study and, respectively, 4.35 ± 0.73 mm 1 month after therapy and 2.82 ± 0.43 (p> 0.05 ) 3 months after therapy. The mean value of clinical attachment level (CAL) at baseline in the study group was 5.84± 0.79 mm, 1 month after therapy it was was 4.92 ±0.71 mm. and 3 month after therapy it was3,4 ±0,7. Therefore, in periodontitis patients; there was a statistically significant decrease in mean CAL post-operatively (at P < 0.001).The mean value of osteocalcin in serum at baseline was 10.58 ng/ml, 1 month after periodontal therapy it was 14.23 ng/ml, 3 month after periodontal therapy it was was 17.74 ng/ml. As a result, there is a statistically significant increase in mean osteocalcin postoperatively (at P < 0.001).The study results showed that elevated serum osteocalcin concentration in patients with chronic periodontitis after periodontal therapy correlates with PPD and CAL, suggesting that its role could be used as a diagnostic test for patient and to complement other diagnostic tests. Conclusions: The results of study showed that a combination treatment with the use of bone graft materials, hyaluronic acid (Gengigel®) and magnetic laser therapy is an effective therapy for the treatment of periodontal disease.