Research and implementation of energy crops using short-rotation woody species (SRWC) are increasing in Latin America, especially for biomass production and use in bioenergy. For this purpose, one of the main factors to consider is species assessment. Therefore, the objective of the present study was to evaluate the growth characteristics, the production and distribution of biomass, and the chemical, physical, and energetic properties of the biomass of three-year-old Dipteryx panamensis plantations in SRWC in three different spacings (1.0x2.0 m, 1.0x0.5 m and 1.0x1.0 m) and establish the best spacing condition for this tropical species. The results showed that the production of biomass varies between 1.1 and 42.36 t.ha-1, and that 33-44% of the production are concentrated in the leaves. At three years, the SRWC presented 50% mortality, with a diameter of 4.8 cm at 30 cm-height from the ground, with total tree heights ranging from 5.17 to 6.98 meters. The evaluation of the biomass showed a calorific value between 18.9 and 19.4 MJ/kg, less than 1.81% of ash content and 86% of volatile content. As for the effect of the spacing, the green density of the wood and the moisture content increased with increasing spacing, while spacings of 1.0x1.0 m and 1.0x0.5 m showed the best behaviour regarding annual biomass production.