ABSTRACT. Hemiodus orthonops is a small fish of the Hemiodontidae family, order Characiformes, with a maximum of 25 cm standard length. Until recently, H. orthonops was an endemic species from the Paraná-Paraguay basin and it was absent from the upper Paraná River basin. Since 2008, it has started to be collected in the upper Paraná River, representing up to 10% of catches. Two population samples of H. orthonops from two localities of the upper Parana River basin (Porto Camargo and Porto Figueira) were analyzed using the allozymes electrophoresis technique. Twenty-one enzymatic loci were detected. The population sample from Porto Camargo displayed a genetic variability (He = 0.1061) higher than that from Porto Figueira (He = 0.0580) and homozygote excess in both of them. The F ST value (0.2081) indicated genetic structure. The excess of homozygotes in both samples was probably due to founder effect in the population.Keywords: Allozymes, fish, genetic distance, heterozygosity, protein polymorphism.Variabilidade genética e evidência do efeito do fundador em Hemiodus orthonops (Characiformes: Hemiodontidae) da bacia do alto rio Paraná, Brasil
O gene DGAT1 é um gene candidato envolvido com o conteúdo de gordura no leite. Neste estudo foram analisadas amostras de DNA de 72 bovinos leiteiros de uma população parcialmente fechada através amostras de sangue. O DNA foi extraído utilizando CTAB e proteinase K. A região alvo do gene foi amplificada por PCR e os resultados foram visualizados com a utilização de gel de poliacri-lamida. Na população em estudo foi verificada a presença de quatro alelos, cujas frequências foram: A= 4,0%, B= 50%, C= 40%, e D= 6,0%. Foi verificado o equilíbrio de Hardy-Weinberg através do teste de c², concluindo-se que a população não se encontrava em equilíbrio para o locos avaliado.
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