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Environment material was a kind of material that considering the environment and ecology protection. Microbe as the key material of sewage treatment system played an important role in environmental protection. The paper researched that a strain of denitrifying phosphorus-removing bacteria could be isolated and screened from dephosphorization system by the conventional microbial breeding technology. The strain was preliminarily appraised itself belong to Pseudomonas sp., ultimately named as PX3.