The distributions of HLA allele and haplotype are variable in different ethnic populations and the data for some populations have been published. However, the data on HLA-C and HLA-DQB1 loci and the haplotype of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci at a high-resolution level are limited in Zhejiang Han population, China. In this study, the frequencies of the HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci and haplotypes were analysed among 3,548 volunteers from the Zhejiang Han population using polymerase chain reaction sequencing-based typing method. Totals of 51 HLA-A, 97 HLA-B, 45 HLA-C, 53 HLA-DRB1 and 27 HLA-DQB1 alleles were observed.The top three frequent alleles of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci were Ahaplotypes with a frequency of ≥0.1% were found and the haplotypes with frequency greater than 3% were Ahood ratios test for the linkage disequilibrium of two loci haplotypes was revealed that the majority of the pairwise associations were statistically significant. The data presented in this study will be useful for searching unrelated HLA-matched donor, planning donor registry and for anthropology studies in China. K E Y W O R D Sallele frequency, human leucocyte antigen, polymerase chain reaction sequencing-based typing

The distributions of HLA allele and haplotype are various in the populations. Currently, the data for HLA alleles and haplotypes at three fields resolution level in Chinese Han population is rare. Here, the HLA alleles and haplotypes of the 1734 cord blood samples from Zhejiang Han population, China were reported at three fields resolution. All samples were randomly collected from the Zhejiang Cord Blood Bank, China. HLA-A, -B, -C, -DRB1, -DQB1, -DRB3/4/5 loci was genotyped using next generation sequencing method. The genotypes of the samples were assigned using the HLA TypeStream Visual Software version 1.2.0. The frequency of alleles, haplotype estimation and linkage disequilibrium analysis were performed with the Arlequin software 3.5.2.2. It was found that the top three frequent alleles of HLA-

s Ca 3 M 2 Ge 3 O 12 :Mn 2+ ,Mn 4+ (M = Al, Ga) with sufficient red light component were developed, and their lattice occupancy and luminescent properties of different color centers have been studied. In Ca 3 M 2 Ge 3 O 12 :Mn, the abnormal self-reduction of Mn 4+ to Mn 2+ occurs. The doped manganese ion (using MnO 2 as Mn source) occupies dodecahedron Ca 2+ site forming Mn 2+ (I) color center with orange emission, while it occupies octahedral Al 3+ /Ga 3+ site forming Mn 2+ (II) and Mn 4+ color centers with red and deep red light emission. The thermal quenching properties show that Ca 3 M 2 Ge 3 O 12 :Mn (M = Al, Ga) samples have low thermal quenching, and the thermal quenching of Mn 2+ (I) is lower than that of Mn 2+ (II) due to existence of more defects nearby the Mn 2+ (II) providing additional nonradiative recombination decay path. Ca 3 Al 2 Ge 3 O 12 :Mn has a lower thermal quenching than Ca 3 Ga 2 Ge 3 O 12 :Mn because the former possesses higher rigidity. The obtained results reveal that Ca 3 M 2 Ge 3 O 12 :Mn 2+ ,Mn 4+ (M = Al, Ga) has potential practical value in WLEDs. Moreover, the luminescence of Mn 2+ and Mn 4+ ions in a matrix provides a new strategy for designing and synthesizing new luminescent materials.