WC studled mltogemc slgnal tmnsductlon m normal and oncogene-transformed 32D cells, a murme hematopolehc cell hne that IS normally dependent on mterleukm-3 (IL3) for prohferatlon and survival The formatlon of second messengers was measured m normal cells stimulated with IL3, and m cells transfected with foreign growth factor receptor genes and stimulated with appropriate growth factors We also measured the steady-state lcvcl of second messengers m 32D cells transformed by e&B, abl, and SK oncogencs which abrogate growth factor requirement We found that IL3 stnnulated the formatIon ot dlacylglyccrol independently of mosltol hpld turnover, but concomitantly with Increased turnover of phosphatldylchohnc Epldermal growth factor (EGF), and platelct-derived growth factor (PDGF) stimulated the 'classsal' turnover of mosltol hplds with formahon of dlacylglycerol and c&mm-moblhemg mosltol phosphates Colony stlmulatmg factor-l trigged mosltol lipId turnover, although to a much lower extent than EGF and PDGP Transformed cells showed elevated levels of dlacylglycerol together +i/lEh mcr._ased turnover of phosphomosmdes and phospllatldylchohne Taken togcthcr thcsc results mdlcatc that different growth factors and oncoprotems associate with multiple slgnallmg pathways m 32D cells