In order to solve the problems of high consumption cost and long transportation and distribution cycle of cross-border logistics, a control optimization design method of radio frequency identification technology in the express logistics distribution system of the Internet of Things is proposed. The details of the method are RFID positioning technology, laser ranging technology, RFID and laser fusion positioning feasibility analysis, and moving target state estimation. The experimental results show that when the phase shift threshold φ and the included angle of the antenna are 90°, the error can be reduced to 0.36 m, and the recognition rate can be increased to 94.8%. The simulation results show that, on the premise of meeting the customer’s expectation of timeliness, the actual logistics distribution cost is significantly lower than the customer’s expected logistics distribution cost, which verifies the effectiveness of the proposed method.