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The high-speed railway (HSR) has been a long-term hotspot in both scientific and engineering societies to enhance the long-term high quality HSR service. This study aims to investigate the WJ-7B type small resistance fastener rubber pad applied in HSR, and temperature sweep test is applied to determine the mechanical parameters of the fastener rubber pad, which are hereafter introduced into the vehicle-track-viaduct vertical coupling model via dynamic flexibility method. The track irregularity spectrum is considered as fixed-point excitation to investigate the temperature-dependent effect of fastener rubber pad on the dynamic responses. The results reveal that the rigidity of the fastener rubber pad is low temperature sensitive and high temperature stable, and the temperature variation has little effect on the vertical dynamic responses of the vehicle. The dynamic flexibility of the rail increases in amplitude and the dominant frequency decreases as the temperature of the fastener rubber pad increases. The vertical dynamic responses of the wheel-rail force, the wheelset and the rail-viaduct system gradually decrease as the temperature of the fastener rubber pad increases, and the peak frequency follows the similar rule. While under high temperature circumstances, the temperature dependent stiffness of the fastener rubber pad has little influence on the peak of the dominant frequency in the vertical dynamic response of the track-viaduct system.

This study proposed a novel vibration mitigation slab track plate design to mitigate the vibration induced in urban rail transit operations. The optimal recipe for the newly designed slab track plate is obtained by a series of laboratory tests, and both newly designed vibration mitigation slab track plates and normal slab track plates are fabricated and hereinafter tested. The newly designed slab track plate was examined with a series of laboratory tests in comparison with the normal slab track plate. The PolyMAX method is then adopted for extracting the modal properties, including resonant frequencies, and damping ratios are also determined for both the designed slab and normal slab track plates. A vibration mitigation level (Ls) is defined to address the vibration mitigation performance taking the acceleration response of the normal slab track plate as reference. The newly designed slab track plate demonstrated better dynamic and damping characteristics in comparison with the normal slab track plate. Under the same excitation force, the newly designed slab track plate can mitigate 8.9 dB on average in the frequency range [20, 400] Hz, expressing the feasibility of effective vibration mitigation capacity.

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Metallothioneins (MTs) as reactive oxygen species (ROS) scavengers play important roles in stress response and heavy metal homeostasis. In Hevea brasiliensis (the para rubber tree that is the source of commercial natural rubber) and in other trees, the functions of MTs are not well understood. Latex exudes when the rubber tree is tapped. The flow of latex and its regeneration can be enhanced by tapping, wounding and ethylene treatment, all of which produce ROS as a by-product. Here, we show the presence of four MT genes in H. brasiliensis, comprising three Type 2 (HbMT2, -2a and -2b) and one Type 3 (HbMT3L) isoforms, representing one of the smallest MT gene families among angiosperms. The four HbMTs exhibited distinct tissue expression patterns: HbMT2 and HbMT3L mainly in leaves, HbMT2a specifically in flowers and HbMT2b in diverse tissues. The expression of HbMT2b, an isoform present in latex, decreased significantly in the latex following the stress-inducing treatments of tapping, wounding and ethephon (an ethylene generator). The expressions of the leaf-abundant isoforms, HbMT2 and -3L were up-regulated following pathogenic fungus infection and high-temperature stress, but down-regulated by low-temperature stress. These reactions were consistent with multiple defense- and hormone-responsive cis-acting elements in the HbMT promoters. Nine transcription factors were shown to implicate in the high-temperature responsiveness of HbMT2 and -3L in leaves. Overexpression of HbMT2 in Escherichia coli enhanced the bacterium's tolerance to heavy metals and ROS, consistent with its predicted role as an ROS scavenger. Taken together, our results, along with other relevant studies, suggest an important role of HbMTs in latex regeneration as well as species adaptation via the regulation of ROS homeostasis.