Despite a long and successful experience with the use of pumpkin seed oil (PSO) in patients with chronic prostatitis (CP), the pharmacodynamics of PSO is not fully understood. We hypothesized that pumpkin seeds and PSO contain pharmacologically active substances that are able to influence on the rate of oxygen consumption (ROC) by the prostate tissue, which may be a determinant of their effectiveness in men with CP. The aim: to study the effect of eating pumpkin seeds, containing PSO, on the ROC of the prostate tissue in white outbred rats. Materials and methods. The control group consisted of 16 outbred male rats. The experimental group consisted of 6 white outbred male rats, which on the eve received, in addition to the standard diet, pumpkin seeds without restriction. Thus, 22 adult outbred male rats were used in the study. The ROC of the prostate tissue was measured by an optical sensor of dissolved oxygen with a DKTP-03 thermoelectric converter. A prostatectomy was performed under anesthesia. Immediately after that, prostate tissue weighing 0.5 g was placed in a beaker, filled with 15.0 ml of 0.9% sodium chloride solution at a temperature of 36.6 °C. An optical oxygen sensor DKTP-03 connected to an oxygen analyzer "Expert-009" was placed in a beaker and the tightness was created. The analyzer showed the concentration of dissolved molecular oxygen (CDO2) in a 0.9% solution of sodium chloride, expressed in mg / l. We recorded the CDO2 readings every minute from the beginning of the experiments (0 minutes) to their end (60 minutes). Results and conclusion. we have found for the first time that PSO statistically significantly increases the ROC in rat prostate tissue (p < 0.05).

Background. For objective assessment of the functional status of prostatic tissue, the «Kalium Parameter of Laboratory analysis, Evaluating in Ejaculate (KAPLEE)» was offered. KAPLEE is the concentration of K+ ions, measuring with the help of potentiometry in 42 ml of water solution, which contains 1 ml of ejaculate, 39 ml of distilled water and 2 ml of special buffer solution. The aim: to check the hypothesis that the deviation of KAPLEE from the normal range towards reduction or towards increase is the determinant of less efficacy of pumpkin seed oil (PSO) in patients with chronic prostatitis 3B (CP3B). Materials and methods: In trial 51 patients with the diagnosis of CP3B, who received PSO 5400 mg a day (4 capsules 3 times a day) for 30 days as monotherapy, took part. The study included 2 stages. During first stage each patient evaluated the efficacy of this pharmacotherapy in his case; it varied from 1 point (absolute non-efficacy) to 5 points (absolute efficacy). Then each patient ejaculated into container, and the concentration of KAPLEE was measured. Thus, the data of each patient were placed in two-axial system of coordinates: axis of abscisses was the concentration of KAPLEE, axis of ordinates was the mark of efficacy. Then the cluster analysis of these data was made. Results and conclusion. Concentration of potassium ions in the sperm can be considered a determinant of clinical effectiveness of pumpkin seed oil in patients with chronic prostatitis.

Abstract. Background. Previously, we first formulated the concept of targeted prostatic pharmaconecrosis (TPPhN). TPPhN is the death of those areas of the prostate tissue, that are proven or suspected to contain tumor cells of prostate cancer, due to the direct injection of a pharmacologically active substance into the prostate tissue. The aim: to study whether TPPhN occurs in the prostate tissue of male rats three hours after injection of 0.05 ml noradrenaline hydrotartrate (NH) solution into it. Materials and methods. The experiments were carried out on 12 outbred male rats aged 10 months. The animals were divided into two groups of six individuals each. Under anesthesia, the rats of the first group received only the injection of 0.05 ml 0,2% solution of NH into the left lobe of the prostate. Rats of the second group first received doxazosin 0.00025 g intragastrically, and then an intraprostatic injection of 0.05 ml of a 0.2 % solution of NH into the left lobe of the prostate was made. 3 hours after intraprostatic injection, macroscopic changes in the left lobe of the prostate were assessed, then the left lobe was resected. Results and conclusions. In all experiments, a demarcation line was clearly visible, delimiting the focus of TPPhN from intact tissue of the prostate. Typical morphological changes in the prostate tissue in the focus of TPPhN were studied. Close attention was paid to the characteristics of the ischemic penumbra between the focus of TPPhN and intact prostate tissue. It was found that in animals of the second group, the penumbra turned out to be clearer and narrower; due to this, the focus of TPPhN was sufficiently sharply delimited from normal prostate tissue. The fact established by us allows us to consider such a combination of substances as promising in the development of the TPPhN technique in humans.