The current study describes a new method for asexual reproduction induction by fission. It results in higher rates of growth, survival and gut regeneration as compared to the conventional technique. Prior to asexual reproduction induction, samples were exposed to chemical evisceration induction through injection of 0.5 ml of 0.45M KCI into perivisceral coelomic cavity. Fission was induced in eviscerated group and normal (non-eviscerated) group through tightening the rubber bands around animal's body at the middle. We compared the modes of fission and the rates of growth, survival and gut regeneration over five months between two groups. We propose a new asexual reproduction induction method in which sea cucumber chemically induced to eviscerate prior to fission induction.