Background The national wide lockdown order imposed in early April 2020 due to the COVID-19 outbreak has complicated mosquito control activities across the United States (US), and Florida is no exception. Mosquito control districts and public health programs are the first line of defense against mosquitoes-borne pathogens in the state of Florida. The purpose of study is to understand how the COVID-19 outbreak has impact the capabilities of mosquito programs to implement key mosquito measures to mitigate emergence and/or re-emergence of arthropod-borne arboviral diseases. Methods In a self-administered online survey, we examined capabilities of all Florida mosquito control programs during the COVID-19 outbreak (both state-approved mosquito districts (N = 63) and public health programs (N = 27). Descriptive statistics were used to summarize information about characteristics of responding mosquito control districts and programs, implemented mosquito control and surveillance activities. We used Bivariate analysis to compare the characteristics of responding mosquito control districts and programs and the self-reported mosquito measures. Results Of the recruited programs, 77 completed the survey (85.6% response rate; 77/90). Of the responding programs, 57.5% (n = 42) were Board of County Commissioners (BOCC) programs, 21.9% (n = 16) were independent tax districts, 13.7% (n = 10) were municipal programs, and only 6.8% (n = 5) were either health or emergency departments. Except for arbovirus surveillance, most programs either fully or partially performed larval (61.8%) and adult (78.9%) surveillance; and for Aedes aegypti (71.9%, n = 46), Aedes albopictus (85.9%, n = 55), Culex quinquefasciatus (88.2%, n = 60), and Culex nigripalpus (90.5%, n = 57). Conclusions Findings underscore the importance of ongoing mosquito control activities and suggests that Florida mosquito control programs are vigilant and have significant capability to handle potential mosquito-borne disease threats, but arbovirus surveillance systems -- laboratory testing of mosquito pools and testing of human and nonhuman specimens for arboviruses are needed during pandemics as well.