The price of spinel has sharply risen in recent years, and its market acceptance is extending. Among the many sources of spinel, Man Sin in Myanmar is the most famous for its bright neon pinkish red to reddish pink color, named “Jedi” spinel commercially. In this paper, spinel samples with the bright neon pinkish red to reddish pink from Man Sin in Myanmar were studied non-destructively with X-ray fluorescence spectrometer (EDXRF), Fourier transform infrared spectrometer (FTIR), fluorescence spectroscopy, a UV-Vis spectrophotometer and Raman spectroscopy. The results show that the samples from Man Sin in Myanmar are the magnesia-alumina spinels. The high content of the red-chromogenic element Cr, an intermediate content of V and Zn, and a low content of the chromogenic element Fe may be responsible for the distinctive neon color of the spinel samples. The presence of Ti may also serve as a basis for the identification of its origin in Man Sin in Myanmar. The fluorescence spectrum analysis of spinels shows them to have multiple excitation peaks in the region 600–800 nm, which are caused by the jump of Cr3+ between the 2E→4A2 inter-energy band lattice. The amount of Zn affects the fluorescence spectrum intensity of spinels. The higher the Zn content, the stronger the fluorescence intensity. The spinel structure is uncomplicated and four more obvious absorption peaks appear in the IR spectrum, and the direction of the absorption peaks is shifted if a homogeneous substitution of the AB2O4 structure occurs; the Raman spectra of spinel samples have four vibrational peaks at 100–2000 cm−1, 310 cm−1, 407 cm−1, 665 cm−1 and 763 cm−1, which has spinel species-identification significance.