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Determinations are carried out In a continuous flow system. Samples pass through a column packed with controlled porosity glass on which the enzyme /3-glucuronldase Is Immobilized; glucuronides are hydrolyzed to glucuronic acid and the corresponding aglycon. The effluent from this column combines with streams containing NaOH, lucigenin (/V,/V'-dlmethyl-9,9'-dlacrldlnlum nitrate), and sodium dodecyl sulfate. Chemiluminescent emission intensity Is proportional to the free glucuronic acid concentration and therefore to the original glucuronide concentration. Glucuronides tested were phenyl, nitrophenyl, methylumbellHeryl, bromonaphthyl, estradiol, and androsterone glucuronide. Detection limits are 5-10 µ , and working curves are linear up to 2 mM. Precision Is 2% relative standard deviation. Other organic reductants, which may be present In biological fluids and would Interfere In chemiluminescent measurements, are eliminated from these samples by HPLC separation on an anion exchange column located between the enzyme column and the addition of chemiluminescent reagents. Urine samples are analyzed by this method and the results compared to a colorimetric analysis.