A gas chromatographic method was developed and validated for the determination of bilobalide and ginkgolides A, B, C and J in Ginkgo biloba L. The Ginkgo terpene trilactones have been dissolved in 57% of pure ethanol in purified water and separated by extraction with a mixture of toluene/ethyl acetate. After evaporation of the solvent with nitrogen and derivatization, these chemical markers have been quantified using GC-FID. The intra-day RSD was ranged from 1.13% for bilobalide to 2.34% in case of ginkgolides. The inter-day RSD was ranged from 2.54% and 3.68% for, bilobalide and ginkgolides respectively. Mean recoveries were 99.78% ± 0.82% and 99.48% ± 0.86% for ginkgolides and bilobalide, respectively. Based on chromatograms, the response of the terpene trilactones was linearly dependent on the terpene concentration, i.e. linear calibration curve was found in the range of the method and was applied to analyze standard extract (containing 5-7% total terpenoids and 22-25% total flavonoids) as working standard and for the analysis of six Ginkgo pharmaceutical products offered on the market.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.